biotinylated goat Search Results


cat ba 9401  (Vector Laboratories)
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vector laboratories ba 1000  (Vector Laboratories)
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goat biotinylated anti mouse antibody  (Vector Laboratories)
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biotinylated horse antigoat  (Vector Laboratories)
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biotinylated goat anti mouse immunoglobulin g  (R&D Systems)
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goat anti gdnf polyclonal antibody  (R&D Systems)
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Fig. 2 Expression of <t>GDNF</t> receptors mRNAs in injured motoneurons during the course of postnatal development. (a) Film autoradiography demonstrated that altera- tion of GFRa1 and c-Ret mRNA expression in the injured hypoglossal nucleus. 4P3, 7P3, 8P3, 11P3, 12P3 and 35P3 indicate that rats were axotomized on 4, 7, 8, 11, 12 and 35 days after birth, respectively, and then killed on postoperative day 3. The arrows indicate the axotomized side of nuclei. Marked conversion from down- to up-regulation of GFRa1 mRNA expression in injured side hypoglossal nucleus was observed along with the development, whereas c-Ret mRNA expression in injured motoneurons is up-regulated in both neo- nates and adults. Scale bar ¼ 500 lm. (b) Immunohistochemical demonstrations using anti-GFRa1 (left column) and anti-c- Ret antibody (right column) in injured motoneurons during the course of devel- opment. 3P3, 7P3, 14P3, 42P3 indicate that rats were axotomized on 3, 7, 14 and 42 days after birth, respectively, and killed 3 days after the axotomy. Scale bar ¼ 250 lm. (c) Semi-quantification of GFRa1 and c-Ret mRNA expression in injured motoneurons. Vertical axis indicates the optical intensity ratio (O/C, see Materials and methods) and the horizontal axis indi- cates the axotomized age. Each point shows the average of the intensity of the positive signal ratios and error bars indicate SE. The dots in the shaded area of the graph mean its expression in injured moto- neuron was down-regulated (O/C < 1).
Goat Anti Gdnf Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated goat polyclonal anti bmp 2 4  (R&D Systems)
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Fig. 2 Expression of <t>GDNF</t> receptors mRNAs in injured motoneurons during the course of postnatal development. (a) Film autoradiography demonstrated that altera- tion of GFRa1 and c-Ret mRNA expression in the injured hypoglossal nucleus. 4P3, 7P3, 8P3, 11P3, 12P3 and 35P3 indicate that rats were axotomized on 4, 7, 8, 11, 12 and 35 days after birth, respectively, and then killed on postoperative day 3. The arrows indicate the axotomized side of nuclei. Marked conversion from down- to up-regulation of GFRa1 mRNA expression in injured side hypoglossal nucleus was observed along with the development, whereas c-Ret mRNA expression in injured motoneurons is up-regulated in both neo- nates and adults. Scale bar ¼ 500 lm. (b) Immunohistochemical demonstrations using anti-GFRa1 (left column) and anti-c- Ret antibody (right column) in injured motoneurons during the course of devel- opment. 3P3, 7P3, 14P3, 42P3 indicate that rats were axotomized on 3, 7, 14 and 42 days after birth, respectively, and killed 3 days after the axotomy. Scale bar ¼ 250 lm. (c) Semi-quantification of GFRa1 and c-Ret mRNA expression in injured motoneurons. Vertical axis indicates the optical intensity ratio (O/C, see Materials and methods) and the horizontal axis indi- cates the axotomized age. Each point shows the average of the intensity of the positive signal ratios and error bars indicate SE. The dots in the shaded area of the graph mean its expression in injured moto- neuron was down-regulated (O/C < 1).
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biotinylated polyclonal goat antinrp1 igg  (R&D Systems)
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Fig. 2 Expression of <t>GDNF</t> receptors mRNAs in injured motoneurons during the course of postnatal development. (a) Film autoradiography demonstrated that altera- tion of GFRa1 and c-Ret mRNA expression in the injured hypoglossal nucleus. 4P3, 7P3, 8P3, 11P3, 12P3 and 35P3 indicate that rats were axotomized on 4, 7, 8, 11, 12 and 35 days after birth, respectively, and then killed on postoperative day 3. The arrows indicate the axotomized side of nuclei. Marked conversion from down- to up-regulation of GFRa1 mRNA expression in injured side hypoglossal nucleus was observed along with the development, whereas c-Ret mRNA expression in injured motoneurons is up-regulated in both neo- nates and adults. Scale bar ¼ 500 lm. (b) Immunohistochemical demonstrations using anti-GFRa1 (left column) and anti-c- Ret antibody (right column) in injured motoneurons during the course of devel- opment. 3P3, 7P3, 14P3, 42P3 indicate that rats were axotomized on 3, 7, 14 and 42 days after birth, respectively, and killed 3 days after the axotomy. Scale bar ¼ 250 lm. (c) Semi-quantification of GFRa1 and c-Ret mRNA expression in injured motoneurons. Vertical axis indicates the optical intensity ratio (O/C, see Materials and methods) and the horizontal axis indi- cates the axotomized age. Each point shows the average of the intensity of the positive signal ratios and error bars indicate SE. The dots in the shaded area of the graph mean its expression in injured moto- neuron was down-regulated (O/C < 1).
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room temperature rabbit igg biotin goat pab e0432 dakocytomation denmark a s goat igg biotin donkey pab baf109 r d systems open  (R&D Systems)
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Fig. 2 Expression of <t>GDNF</t> receptors mRNAs in injured motoneurons during the course of postnatal development. (a) Film autoradiography demonstrated that altera- tion of GFRa1 and c-Ret mRNA expression in the injured hypoglossal nucleus. 4P3, 7P3, 8P3, 11P3, 12P3 and 35P3 indicate that rats were axotomized on 4, 7, 8, 11, 12 and 35 days after birth, respectively, and then killed on postoperative day 3. The arrows indicate the axotomized side of nuclei. Marked conversion from down- to up-regulation of GFRa1 mRNA expression in injured side hypoglossal nucleus was observed along with the development, whereas c-Ret mRNA expression in injured motoneurons is up-regulated in both neo- nates and adults. Scale bar ¼ 500 lm. (b) Immunohistochemical demonstrations using anti-GFRa1 (left column) and anti-c- Ret antibody (right column) in injured motoneurons during the course of devel- opment. 3P3, 7P3, 14P3, 42P3 indicate that rats were axotomized on 3, 7, 14 and 42 days after birth, respectively, and killed 3 days after the axotomy. Scale bar ¼ 250 lm. (c) Semi-quantification of GFRa1 and c-Ret mRNA expression in injured motoneurons. Vertical axis indicates the optical intensity ratio (O/C, see Materials and methods) and the horizontal axis indi- cates the axotomized age. Each point shows the average of the intensity of the positive signal ratios and error bars indicate SE. The dots in the shaded area of the graph mean its expression in injured moto- neuron was down-regulated (O/C < 1).
Room Temperature Rabbit Igg Biotin Goat Pab E0432 Dakocytomation Denmark A S Goat Igg Biotin Donkey Pab Baf109 R D Systems Open, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated goat polyclonal igg  (R&D Systems)
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Fig. 2 Expression of <t>GDNF</t> receptors mRNAs in injured motoneurons during the course of postnatal development. (a) Film autoradiography demonstrated that altera- tion of GFRa1 and c-Ret mRNA expression in the injured hypoglossal nucleus. 4P3, 7P3, 8P3, 11P3, 12P3 and 35P3 indicate that rats were axotomized on 4, 7, 8, 11, 12 and 35 days after birth, respectively, and then killed on postoperative day 3. The arrows indicate the axotomized side of nuclei. Marked conversion from down- to up-regulation of GFRa1 mRNA expression in injured side hypoglossal nucleus was observed along with the development, whereas c-Ret mRNA expression in injured motoneurons is up-regulated in both neo- nates and adults. Scale bar ¼ 500 lm. (b) Immunohistochemical demonstrations using anti-GFRa1 (left column) and anti-c- Ret antibody (right column) in injured motoneurons during the course of devel- opment. 3P3, 7P3, 14P3, 42P3 indicate that rats were axotomized on 3, 7, 14 and 42 days after birth, respectively, and killed 3 days after the axotomy. Scale bar ¼ 250 lm. (c) Semi-quantification of GFRa1 and c-Ret mRNA expression in injured motoneurons. Vertical axis indicates the optical intensity ratio (O/C, see Materials and methods) and the horizontal axis indi- cates the axotomized age. Each point shows the average of the intensity of the positive signal ratios and error bars indicate SE. The dots in the shaded area of the graph mean its expression in injured moto- neuron was down-regulated (O/C < 1).
Biotinylated Goat Polyclonal Igg, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated normal goat igg  (R&D Systems)
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Fig. 2 Expression of <t>GDNF</t> receptors mRNAs in injured motoneurons during the course of postnatal development. (a) Film autoradiography demonstrated that altera- tion of GFRa1 and c-Ret mRNA expression in the injured hypoglossal nucleus. 4P3, 7P3, 8P3, 11P3, 12P3 and 35P3 indicate that rats were axotomized on 4, 7, 8, 11, 12 and 35 days after birth, respectively, and then killed on postoperative day 3. The arrows indicate the axotomized side of nuclei. Marked conversion from down- to up-regulation of GFRa1 mRNA expression in injured side hypoglossal nucleus was observed along with the development, whereas c-Ret mRNA expression in injured motoneurons is up-regulated in both neo- nates and adults. Scale bar ¼ 500 lm. (b) Immunohistochemical demonstrations using anti-GFRa1 (left column) and anti-c- Ret antibody (right column) in injured motoneurons during the course of devel- opment. 3P3, 7P3, 14P3, 42P3 indicate that rats were axotomized on 3, 7, 14 and 42 days after birth, respectively, and killed 3 days after the axotomy. Scale bar ¼ 250 lm. (c) Semi-quantification of GFRa1 and c-Ret mRNA expression in injured motoneurons. Vertical axis indicates the optical intensity ratio (O/C, see Materials and methods) and the horizontal axis indi- cates the axotomized age. Each point shows the average of the intensity of the positive signal ratios and error bars indicate SE. The dots in the shaded area of the graph mean its expression in injured moto- neuron was down-regulated (O/C < 1).
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h incubation  (R&D Systems)
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Fig. 2 Expression of <t>GDNF</t> receptors mRNAs in injured motoneurons during the course of postnatal development. (a) Film autoradiography demonstrated that altera- tion of GFRa1 and c-Ret mRNA expression in the injured hypoglossal nucleus. 4P3, 7P3, 8P3, 11P3, 12P3 and 35P3 indicate that rats were axotomized on 4, 7, 8, 11, 12 and 35 days after birth, respectively, and then killed on postoperative day 3. The arrows indicate the axotomized side of nuclei. Marked conversion from down- to up-regulation of GFRa1 mRNA expression in injured side hypoglossal nucleus was observed along with the development, whereas c-Ret mRNA expression in injured motoneurons is up-regulated in both neo- nates and adults. Scale bar ¼ 500 lm. (b) Immunohistochemical demonstrations using anti-GFRa1 (left column) and anti-c- Ret antibody (right column) in injured motoneurons during the course of devel- opment. 3P3, 7P3, 14P3, 42P3 indicate that rats were axotomized on 3, 7, 14 and 42 days after birth, respectively, and killed 3 days after the axotomy. Scale bar ¼ 250 lm. (c) Semi-quantification of GFRa1 and c-Ret mRNA expression in injured motoneurons. Vertical axis indicates the optical intensity ratio (O/C, see Materials and methods) and the horizontal axis indi- cates the axotomized age. Each point shows the average of the intensity of the positive signal ratios and error bars indicate SE. The dots in the shaded area of the graph mean its expression in injured moto- neuron was down-regulated (O/C < 1).
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Image Search Results


Fig. 2 Expression of GDNF receptors mRNAs in injured motoneurons during the course of postnatal development. (a) Film autoradiography demonstrated that altera- tion of GFRa1 and c-Ret mRNA expression in the injured hypoglossal nucleus. 4P3, 7P3, 8P3, 11P3, 12P3 and 35P3 indicate that rats were axotomized on 4, 7, 8, 11, 12 and 35 days after birth, respectively, and then killed on postoperative day 3. The arrows indicate the axotomized side of nuclei. Marked conversion from down- to up-regulation of GFRa1 mRNA expression in injured side hypoglossal nucleus was observed along with the development, whereas c-Ret mRNA expression in injured motoneurons is up-regulated in both neo- nates and adults. Scale bar ¼ 500 lm. (b) Immunohistochemical demonstrations using anti-GFRa1 (left column) and anti-c- Ret antibody (right column) in injured motoneurons during the course of devel- opment. 3P3, 7P3, 14P3, 42P3 indicate that rats were axotomized on 3, 7, 14 and 42 days after birth, respectively, and killed 3 days after the axotomy. Scale bar ¼ 250 lm. (c) Semi-quantification of GFRa1 and c-Ret mRNA expression in injured motoneurons. Vertical axis indicates the optical intensity ratio (O/C, see Materials and methods) and the horizontal axis indi- cates the axotomized age. Each point shows the average of the intensity of the positive signal ratios and error bars indicate SE. The dots in the shaded area of the graph mean its expression in injured moto- neuron was down-regulated (O/C < 1).

Journal: Journal of neurochemistry

Article Title: Developmental alteration of nerve injury induced glial cell line-derived neurotrophic factor (GDNF) receptor expression is crucial for the determination of injured motoneuron fate.

doi: 10.1046/j.1471-4159.2002.01043.x

Figure Lengend Snippet: Fig. 2 Expression of GDNF receptors mRNAs in injured motoneurons during the course of postnatal development. (a) Film autoradiography demonstrated that altera- tion of GFRa1 and c-Ret mRNA expression in the injured hypoglossal nucleus. 4P3, 7P3, 8P3, 11P3, 12P3 and 35P3 indicate that rats were axotomized on 4, 7, 8, 11, 12 and 35 days after birth, respectively, and then killed on postoperative day 3. The arrows indicate the axotomized side of nuclei. Marked conversion from down- to up-regulation of GFRa1 mRNA expression in injured side hypoglossal nucleus was observed along with the development, whereas c-Ret mRNA expression in injured motoneurons is up-regulated in both neo- nates and adults. Scale bar ¼ 500 lm. (b) Immunohistochemical demonstrations using anti-GFRa1 (left column) and anti-c- Ret antibody (right column) in injured motoneurons during the course of devel- opment. 3P3, 7P3, 14P3, 42P3 indicate that rats were axotomized on 3, 7, 14 and 42 days after birth, respectively, and killed 3 days after the axotomy. Scale bar ¼ 250 lm. (c) Semi-quantification of GFRa1 and c-Ret mRNA expression in injured motoneurons. Vertical axis indicates the optical intensity ratio (O/C, see Materials and methods) and the horizontal axis indi- cates the axotomized age. Each point shows the average of the intensity of the positive signal ratios and error bars indicate SE. The dots in the shaded area of the graph mean its expression in injured moto- neuron was down-regulated (O/C < 1).

Article Snippet: Goat anti-GDNF polyclonal antibody (1 : 50; R & D Systems, Minneapolis, MN, USA), biotinylated goat anti-GFRa1 polyclonal antibody (1 : 50, R & D) and rabbit anti-c-Ret polyclonal antibody, R787 (1 : 50, IBL, Fujioka, Japan) were used for detection of GDNF, GFRa1 and c-Ret, respectively.

Techniques: Expressing, Autoradiography, Immunohistochemical staining